The Development of Low - Speed " A " Series
نویسندگان
چکیده
The evolution of a series of low-speed zonal centrifuge rotors for the separation of cells, bacteria, and subcellular particles visible in the light microscope {Rotors A-V, VI, IX, and XII) is described. All are dynamically loaded and unloaded and may be used for either rate-zonal or isopycnic-zonal separations. Transparent plastic end caps allow direct observation of the separations. The last rotor of the series (AXII) has a total rotor volume of 1400 ml and a maximum radius of17.78 cm. The sample zone volume can be as large as 50 ml, and the density"gradient volume is usually one liter. Experimental studies with model particles demonstrate that narrow starting zones and useful separations can be obtained.Nat Cancer Inst Monogr 21: 113-136, 1966.
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تاریخ انتشار 2009